Re. Is milk protein Y-phosphorylated?

JONG, SONG-MUH J SJJ at icbr.ifas.ufl.edu
Fri Jan 21 02:52:53 EST 1994


From: "Curtis L. Ashendel" <ashendel at aclcb.purdue.edu>
>You will (IMHO) never get a figure published in the reviewed literature if 
>it is of an anti-pY blot done with milk as a blocking agent. 

I found this statement very unfounded. We have been using non-fat
dry milk as the blocking agent for anti-phosphotyrosine immunoblots
and have not had much problem with the background.


Song-Muh Jong
sjj at icbr.ifas.ufl.edu





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