Re. Is milk protein Y-phosphorylated?
JONG, SONG-MUH J
SJJ at icbr.ifas.ufl.edu
Fri Jan 21 02:52:53 EST 1994
From: "Curtis L. Ashendel" <ashendel at aclcb.purdue.edu>
>You will (IMHO) never get a figure published in the reviewed literature if
>it is of an anti-pY blot done with milk as a blocking agent.
I found this statement very unfounded. We have been using non-fat
dry milk as the blocking agent for anti-phosphotyrosine immunoblots
and have not had much problem with the background.
sjj at icbr.ifas.ufl.edu
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