Your thoughts on Hot PCR for microsatellite studies

Dimitri Odorico chdmo at marlin.jcu.edu.au
Sun Jan 23 19:54:22 EST 1994


I've just reached the stage of doing hot pcr work and i'd like peoples
opinions and/or methods on the matter. Are most people 5'end labelling
their  primers for the pcr or are you directly incorporating labelled
nucleotides in the pcr recation?  

Thanks 
Dimitri Odorico 



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