Your thoughts on Hot PCR for microsatellite studies

Dimitri Odorico chdmo at
Sun Jan 23 19:54:22 EST 1994

I've just reached the stage of doing hot pcr work and i'd like peoples
opinions and/or methods on the matter. Are most people 5'end labelling
their  primers for the pcr or are you directly incorporating labelled
nucleotides in the pcr recation?  

Dimitri Odorico 

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