Coupling DNA to Sephadex?

Leonard N. Bloksberg bloksber at pilot.msu.edu
Mon Jan 24 11:37:00 EST 1994


In Article <ed_Beaty-170194190445 at maloy2.life.uiuc.edu> "ed_Beaty at qms1.life.uiuc.edu (edbeaty)" says:
> Hello!  Here's my problem:  I need to couple DNA, about 200 bp in size, to
> a solid substrate for affinity chromatography.  The protocols I have use
> cyanogen bromide to activate CL-2B Sepharose (Pharmacia) for coupling. 
> Unfortunately, I don't have any Sepharose, but I do have about a dozen
> different grades of Sephadex.  I'm not familiar enough with the chemistry
> involved to know whether I can use a similar protocol to couple my DNA to
> the Sephadex or not.  Does anyone out there have any protocols, or other
> ideas?  (Besides asking Pharmacia, which I'll do on Tuesday.)
> Thanks!
> 
> -Ed Beaty
> Ed_Beaty at qms1.life.uiuc.edu
> 
Just buy some Sepharose.  Sigma sells re-packaged Pharmacia Sepharose CL2B
in smaller (hence cheaper) alliquots.  This tip came to me directly from 
the Pharmacia Technical service rep.  Good Luck
.
	Leonard N. Bloksberg
	bloksber at pilot.msu.edu
.
.
.



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