Re. Bacterial strains for electroporation

JONG, SONG-MUH J SJJ at icbr.ifas.ufl.edu
Tue Jan 25 15:05:07 EST 1994


From: paul-b at molbio.cbs.umn.edu (Paul Bucciaglia) wrote:
>What strains can you all reccommend for electroporation OTHER
>THAN MC1061.  Mc1061 carries streptomycin resistance which is 
>also the selection for an Agrobacterium construct that we work
>with.  I have tried JM101 but got much lower competance.  How
>about Dh5, HB101 or other suggestions?

I found that C600 gave 10 times higher efficiency than XL1-Blue
in electroporation.

Song-Muh Jong
sjj at icbr.ifas.ufl.edu






More information about the Methods mailing list