nash effect with phagemids
nash at nrcbsa.bio.nrc.ca
Tue Jan 25 11:01:24 EST 1994
In article <2i2oaa$17n at mserv1.dl.ac.uk>,
<suter at VAX.mpiz-koeln.mpg.d400.de> wrote:
>John Nash wrote:
>I cannot recall the above described Nash effect taking place if
Yesterday was a *BAD* day! Remind me never to post when I'm having
one of those days... My silly-bone gets stimulated.
About fd ss-ori lowering electroporation frequencies in some hosts...
I've never noticed it when using chemical transformation methods.
Also, I've only used DH5alpha and DH5alpha F' for electroporation, so
I can't say if it is host-dependent.
Also, Clemens, you're right; phagemids are very cumbersome vectors, I
much prefer using pUC18&19. Sometimes I wonder why I gave up
John Nash (nash at nrcbsa.bio.nrc.ca)
Institute for Biological Sciences, National Research Council of Canada,
Yet another Aussie-in-exile ;-)
*** Disclaimer: All opinions are mine, not NRC's! ***
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