iron ions in dna

john hachey HACHEY at ABRSLE.AGR.CA
Wed Jan 26 18:03:35 EST 1994


Date sent:  26-JAN-1994 15:57:27 


>	We have done Phenol/Chloroform DNA extractions on mouse liver
>and have produced pretty brown DNA,  presumably iron.  The DNA has
>been chloroform extracted twice with no further improvement ;-(
>
>	What we want to know is:-
>
>1)	Will the iron affect the numerous PCR's we're going to 
>hit them with?
>
>2)	How can we remove the iron from the DNA,  and how can we 
>avoid this in in future extractions?  (Any good iron chelating agents
>out there?)
>

I believe diethyldithiocarbamic acid (DIECA) chelates iron.
Try it at about 0.1%


>Michael
>
>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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