nash effect with phagemids

the End jgraham at
Wed Jan 26 18:03:38 EST 1994

I have not found the phagemids pBluescript, pTZ18-19, nor any of the many
derivatives I've made from them nor the PSC101 vectors I've made with 
F1 origins to be at all cumbersome in terms of cloning. Do you guys see
low ligation or transformation efficiencies after ligation ? A single-strand
origin has only been an asset in my hands.

J. Graham
Biology and Chemistry Departments

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