ECL Quantitation of westerns

JOHNSON_T johnson_t at
Wed Jan 26 10:06:44 EST 1994

I have a question about quantitiation of western blots 
using chemilluminescence.  I have seen papers which have 
used ECL and densitometry for quantitation of specific 
proteins, but I have doubts about the validity of this 
particular procedure.  I guess if the antibody is 
carefully titrated with known amounts of purified 
antigen, then one could accept the results.  However, 
known amounts of antigen are not always available.  Could 
someone enlighten me on this subject.  I thank you in 

Todd M. Johnson
Sandoz Research Institute
East Hanover, NJ 07936

E-mail  Johnson_T at

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