spotty blots

Wendy Robinson wendy at medgen.unizh.ch
Sat Jan 22 05:30:34 EST 1994


We had spots when we denatured our probes in the reaction volume
even if we mixed the probe well with hybridization solution
before adding to the filter. The spots went away when we boiled
the probe in at least 5ml hybridization solution. I don't know why.



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