buffer for co-immunoprecipitation assay

HAIYAN DENG hdeng at hubio2.harvard.edu
Thu Jan 27 20:52:00 EST 1994

Dear netters,
I am asking for advices on co-immunoprecipitation assay. I am using this
assay to look for protein-protein interaction. It works very well for 
high affinity interaction, but poorly for weaker interaction. I suspect
that the buffer which contains 1% of NP-40 and 0.25% of deoxycholate is 
too stringent.

Does this sound reasonable? And if so, what kind of buffer would you suggest
that would allow me to detect weaker protein-protein interactions?

Thanks in advance.


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