Preparative IEF of proteins

Uttam Rajbhandary bhandary at
Sat Jan 29 17:17:00 EST 1994

In article <2i3791$bf6 at>, LYNDON at BMS-UNIT.ICR.AC.UK writes...
>I am currently in the process of evaluating preparative-scale isoelectric
>focusing methods to purify proteins suitable for crystallisation. Does
>anybody have experience with these methods ... advantages  / disadvantages
>over conventional methods (eg ion exchange, gel permeation chromatography etc)
>and can recommend a system?
>Many thanks

Rotofor by Biorad; found to be quick, and clean in terms of purifying 
proteins.  It's also been suggested that proteins purified by this system 
gives much better crystals.

Dev Mangroo /Bhandary at

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