vaccinia

brett at borcim.wustl.edu brett at borcim.wustl.edu
Thu Jan 27 11:11:52 EST 1994


If your vv/T7 expression is only occuring on the edges of your plates, you
probably are only infecting those cells. You must swirl the inoculum over
the plate frequently before adding media, a general tip. You can test this by
looking for expression of vv antigens via immunoflourescence/staining to show
where your virus is. I predict it will only show up where the b-gal activity
did. Your problem is likely to be in the infection.
brett at borcim.wustl.edu



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