pGEX with NdeI site-help

Blake Smith bsmith at tuzo.erin
Mon Jan 31 17:15:07 EST 1994

Does anybody have a pGEX vector with a NdeI site added in the polycloning
site?  We are having a hard time cloning a fragment into the SmaI site and
were thinking about making a vector with an NdeI site.  We are really
hoping that we can avoid making it ourselves as we are not experts at
cloning ;-)

Please let me know if you can help with this or if you know of another GST
expression vector with a NdeI site.



Blake E. Smith 
bsmith at
Department of Zoology,
University of Toronto

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