PAGE - misformed wells - help

Ding Ming ming at ahabs.wisc.edu
Mon Jan 31 17:26:59 EST 1994


In article <2ijiht$753 at news.bu.edu>, khalid at acs2.bu.edu (Rasheed Khalid)
wrote:


> (sides, bottom or top), there appear what look like bubbles which are
> actually points of separation of gel from the glass plates, and these
> sometimes grow and spread in the area of the clamp like an invasion.
> This happens as polymerisation proceeds. 

This kind of separation happened when you stored the gel for a little bit
longer time and that part of the gel got a little bit dried out, or if you
polymerized the gel in a hurry. I am not sure about the reason and it may
be cause the by the contraction of the gel. But as far as I run into this,
it didn't affect the electrophoresis so I could get along with it.
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Ding Ming                           Telephone: (608)265-3544
University of Wisconsin-Madison          Fax: (608)262-7420

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