Northern blot with ribosomal RNA bands

h-seifert at h-seifert at
Mon Jan 31 15:47:13 EST 1994

In article <wzhou.2.0012F0DC at> wzhou at (Wei Zhou) writes:
>Does any body have a strategy to prevent the non-specific binding of probes to
>ribosomal RNA in Northern Blots?  Thanks.

 We have reduced (but not totally removed) these bands by adding 
an excess of cold rDNA to the hybridization to competatively 
bind up the RNA.  
You'll need to find the proper clone but most rRNA genes are 
readily available.
Good luck
Hank Seifert

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