Northern blot with ribosomal RNA bands

h-seifert at nwu.edu h-seifert at nwu.edu
Mon Jan 31 15:47:13 EST 1994


In article <wzhou.2.0012F0DC at bimcore.cc.emory.edu> wzhou at bimcore.cc.emory.edu (Wei Zhou) writes:
>Does any body have a strategy to prevent the non-specific binding of probes to
>ribosomal RNA in Northern Blots?  Thanks.

 We have reduced (but not totally removed) these bands by adding 
an excess of cold rDNA to the hybridization to competatively 
bind up the RNA.  
You'll need to find the proper clone but most rRNA genes are 
readily available.
Good luck
Hank Seifert




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