Methods January 1994 Archives by author

• Messages sorted by: [ thread ] [ subject ] [ date ]
• More info on this list...

Starting: Sat Jan 1 21:57:49 EST 1994
Ending: Mon Jan 31 23:13:46 EST 1994
Messages: 747

• search for mecillinam, a beta-lactam antibiotic  
• Help: Phage contamination of E.coli growths  
• !!Assistance Request!!   Santhat Lekhavadthanajai - SCMI - 3636187
• How to purify IgM   Santhat Lekhavadthanajai - SCMI - 3636187
• EtBr Staining   Santhat Lekhavadthanajai - SCMI - 3636187
• Thank you for "How to purify IgM"   Santhat Lekhavadthanajai - SCMI - 3636187
• Glycocalyx Extraction   Santhat Lekhavadthanajai - SCMI - 3636187
• Trouble growing EMBL4 to high titer   at reks.uia.ac.be
• Sequencing a hairpin?   A428ENDE at HASARA11.SARA.NL
• Who sells the Tropi-Cooler water bath?   Churward's Lab Account
• digitonin in nuclei isolation buffer - why?   Grizzly Adams
• phone # of Hybaid Ltd.   David A. Adler
• DNA salting-out prep from whole blood?   John Alsobrook
• A sleazy way to avoid direct PCR Sequencing   John Altman
• Some batches of light mineral oil can kill your PCR or RAPD reactions!   Paul St. Amand
• Dichloromethane vs Chloroform   Roger F. Anderson
• Contaminating plasmid   Anderson at seqvax.caltech.edu
• Kanamycin resistance plasmids   Kristin B. Andersson
• Address for American Diagnostica Inc.   Zeno Apostolides
• Antibodies to plant proteins   Zeno Apostolides
• Ab for PPO plant PPO and POD enzymes   Zeno Apostolides
• Multiple DNA sequence alignment   Dean R. Appling
• Iron ions in my DNA   Arild.Tangeras at pki.UiB.no
• Purifying Chicken IgY   Curtis L. Ashendel
• Is milk protein Y-phosphorylated?   Curtis L. Ashendel
• Is milk protein Y-phosphorylated?   Curtis L. Ashendel
• Is milk protein Y-phosphorylated?   Curtis L. Ashendel
• Centrifuge tubes   Graham Atherton
• Oligo cloning   Graham Atherton
• About cycle sequencing   BHWFDUNG at cphkvx.cphk.hk
• Direct sequencing of PCR products   BHWFDUNG at cphkvx.cphk.hk
• Multi-Functional CAT & Luciferase Reporter Gene Vectors   Ram Balaraman
• DNA heterozygosity   Dirk Balfanz
• Plasmid Prep for Sequencing (Sorry if this is FAQ)   Hinayana Bawagan
• Very low pH buffer   Hinayana Bawagan
• DNA conc by EtBr staining?   Hinayana Bawagan
• Magic minipreps--DNA don't cut   Hinayana Bawagan
• DEAE dextran transfection of RNA into a "delicate" cell line   Hinayana Bawagan
• Ligation - dephosphorylation?   Hinayana Bawagan
• PCR Restriction Site engineering   Hinayana Bawagan
• Problem: aggregation of bact. expressed proteins.   Hinayana Bawagan
• Mass spec services?   Clifford J Beall
• Is M13 cloning mutagenic?   Michael Benedik
• Purifying Chicken IgY   David M. Berman
• Need address for A. Polson of Tygerberg RSA   David M. Berman
• Message to anyone from Promega   David M. Berman
• transgenic rats   David M. Berman
• octopine synthase   Berschic at convex.rz.uni-duesseldorf.de
• Solid-state library amplification   Dr. S. Bhattacharya
• Zinc in media   Dr. S. Bhattacharya
• Size fractionating cDNA   Dr. S. Bhattacharya
• Promoter analysis   Sean Buchanan Bioc
• P3 room   Sunee Kertbundit - Dept. of Biochemistry
• Ab purification methods??   Shaun D. Black
• Drosophila DNA preparation method needed   Shaun D. Black
• iron ions in dna   Shaun D. Black
• help: plant nucleus prep   Leonard N. Bloksberg
• 840AV and image analysis   Leonard N. Bloksberg
• Coupling DNA to Sephadex?   Leonard N. Bloksberg
• Multiple DNA sequence alignment   Leonard N. Bloksberg
• linearity of x-ray film/autorads   Leonard N. Bloksberg
• triparental conjgation prblm   Leonard N. Bloksberg
• sequencing of PCR products   Peter T. Boag
• lambda packaging contamination   Bob Barstead, Oklahoma Medical Research Foundation, 825 N.E. 13th Street, Oklahoma City, OK 73104 barsteadr at omrf.uokhsc.edu
• contaminating plasmid   Joyce Bock
• Stratagene Robocycler   Jim Bone
• Fusion vector required!   Bonham, Peta
• Trouble growing EMBL4 to high titer   Toby Bradshaw
• How long is powdered tissue media good for?   John Brunstein
• disregard mailing test   John Brunstein
• looking for ClonmateST   John Brunstein
• easy enzyme assay   John Brunstein
• Contaminating plasmid   John Brunstein
• contaminating plasmids (fwd)   John Brunstein
• Baekon 2000   John Brunstein
• Subcloning with oligos   John Brunstein
• PCR Restriction Site engineering   John Brunstein
• the nash effect/phagemid or pUC ?   John Brunstein
• 50bp PCR Product   John Brunstein
• E. coli strains for electroporation   Paul Bucciaglia
• Multiple DNA sequence alignment   Andrew Bush
• Ericomp thermal cyclers   Tim Buss
• Message to anyone from Promega   Tim Buss
• Subscription   CEINGE at MVX36.CSATA.IT
• Large plaques in lambda libraries   CLONTECH at BIOTECHNET.COM
• pUC118 sequence   CLONTECH at BIOTECHNET.COM
• reply   CLONTECH at BIOTECHNET.COM
• pUC118 & pUC119 sequences   CLONTECH at BIOTECHNET.COM
• Illegitimate transcription   CXSM000
• Help, Actin   CYCS
• Ab purification methods??   CZCT
• Microscopy course   Philip L. Carl
• Scientific clip art   Condie Carmack
• Ericomp thermal cyclers   Lawrence P. Casson
• Need anti-mouse IgG1 (CH1 domain) Mab/hybridoma line   Lawrence P. Casson
• Seeking Mab against CH1 domain of murine IgG   Lawrence P. Casson
• Is milk protein Y-phosphorylated?   George W Chacko
• PCR patent   Alex Chang
• subscription   Lang-Ming Chi
• GST-fusion-protein   Lang-Ming Chi
• Magic minipreps--DNA don't cut   David Chiu
• m.blue staining of DNA   Duncan Clark
• A strain that provides unmethylated lambdaZAP DNA ?   Duncan Clark
• Heat stable E.coli protein   Duncan Clark
• Home made gems don't "invert"   Duncan Clark
• Heat stable E.coli protein   Duncan Clark
• Home made gems don't "invert"   Duncan Clark
• Home made gems don't "invert"   Duncan Clark
• Inducing SOS response   Duncan Clark
• GST fusion prot isolation   Duncan Clark
• Lyophilising Fusarium species   Duncan Clark
• Wheat Germ source for in vitro translation   Sonya Clark
• DNA conc by EtBr staining?   Dr. J.P. Clewley
• DNA/RNA extraction from clotted blood   Dr. J.P. Clewley
• Solid-state library amplification   Michael Coady
• Very low pH buffer   Michael Coady
• spotty blots   Andrew Cockburn
• A sleazy way to avoid direct PCR Sequencing   Andrew Cockburn
• TABLE and SILMUT Programs   DAVIDIAN
• Address for American Diagnostica Inc.   DELLE at IMICILEA.CILEA.IT
• Kanamycin resistance plasmids   DELLE at IMICILEA.CILEA.IT
• Protein/DNA isolation   DELLE at IMICILEA.CILEA.IT
• Biomedical Technol Address wanted   DELLE at IMICILEA.CILEA.IT
• buffer for co-immunoprecipitation assay   HAIYAN DENG
• Contaminating plasmid   Dr R. Dalgleish
• Sequencing a hairpin?   Edwin ten Dam
• MICROINJECTION TECHNIQUES?????   James D DeVault
• easy enzyme assay   Tom Deits
• RT-PCR from few cells.   Greg Denomme
• Home made gems don't "invert"   Greg Denomme
• How to purify IgM   Greg Denomme
• Mass spec services?   Dan Diaz
• Looking for oligo(dU)   Dan Diaz
• QuickHyb   Therese Ann Dietlin
• Construction of TA cloning vector   Dr. C. Dolphin
• transgenic rats   Peter A. Doris
• HPLC columns for fractionating PCR products   Peter A. Doris
• PCR from RNA templates   Marcelo Carnier Dornelas
• Methods on protein-binding sites   Marcelo Carnier Dornelas
• Sequencing gel problems   Andrea Douglas
• Magic minipreps--DNA don't cut   Julian Dow
• Immunoprecipitation of INTACT vesicles (fwd)   MJ Duggan
• Source of C14 labeled EtBr   Patrick G. Durham
• Does Geneclean remove CIP?   Patrick G. Durham
• CIP damages overhangs?   Patrick G. Durham
• **test**   Steve Eklund
• T4PNK 5'-P of primers: pre-PCR cleanup?   Steve Eklund
• DNA isolation formalin fixed tissue   Michael Ell
• help problem with lambda   the End
• nash effect with phagemids   the End
• linearity of x-ray film/autorads   the End
• Protein Sequencing Services (how much are we paying ?)   the End
• DNA software for IBM PCs   FJVAND00 at ukcc.uky.edu
• Bacterial Genetics   Michael John Fath
• AMPLIFY A LIBRARY   Seth Findley
• Amplification of cDNA Library.   Seth Findley
• Strain-Less in Seattle   Seth Findley
• Episome Transfer   Seth Findley
• YEAST TWO-HYBRID//INTERACTION TRAP   Seth Findley
• A sleazy way to avoid direct PCR Sequencing   Seth Findley
• ELECTROPORATION of E. coli   Seth Findley
• New email addresses for MJ Research   Michael Finney
• Proteinase K thawed...AOK??   Karl Fischer
• tobacco acid pyrophosphotase   Parke K. Flick
• Wheat Germ source for in vitro translation   Parke K. Flick
• SUGGESTION ABOUT BUYING A ELECTROPORATION INSTRUMENT   Parke K. Flick
• TABLE and SILMUT Programs   Brian Foley
• HELP! unusual cell growth   Brian Foley
• PCR from RNA templates   Brian Foley
• Plasmid Prep for Sequencing (Sorry if this is FAQ)   Brian Foley
• Help needed to clone a gene   Brian Foley
• Magic minipreps--DNA don't cut   Brian Foley
• Please show me the storage method of HL-60 cell line!.2   Brian Foley
• mammalian transfection/integration   Brian Foley
• Ligation - dephosphorylation?   Brian Foley
• Multiple DNA sequence alignment   Brian Foley
• Multiple DNA sequence alignment   Brian Foley
• Odd problem with sequencing gels   Brian Foley
• ELECTROPORATION of E. coli   Brian Foley
• What does A, C rich sequence mean?   Brian Foley
• Diagnostica Stago: Info wanted   Brian Foley
• Northern blot with ribosomal RNA bands   Brian Foley
• cells in the middle do not express the protein   Brian Foley
• cDNA kits   Brian Foley
• HPLC columns for fractionating PCR products   Brian Foley
• Blocking agents for DIG labelling   Foord, Brett
• Xgal   Foord, Brett
• sequencing of PCR products   Patricia Foster
• Contaminating plasmid   Patricia Foster
• earthquake   Patricia Foster
• optimizing CIP   Patricia Foster
• optimizing CIP   Patricia Foster
• Lysosomal marker   Gaetan.Borgonie at rug.ac.be
• Interpolation methods   Frederick Garbrecht
• GST fusion prot isolation   Frederick Garbrecht
• How to stop plasmids losing inserts?????   Mark D. Garfinkel
• K Ferricyanide   Mark D. Garfinkel
• glucose-transporter Glut-1 antibodies ?   Frank Gaunitz
• Contaminating plasmid   Christian Geltinger
• Contaminating plasmid   Christian Geltinger
• Help Pseudomonas phage   Christian Geltinger
• unmethylated lambdaZAP DNA ?   Steven Russell Genetics
• Mg and PCR   Anna Georgalis
• Inducing SOS response   Dr. Richard V. Goering
• Magic minipreps--DNA don't cut   Anita Gould
• spotty blots   Charles M Griswold
• spotty blots   Charles M Griswold
• spotty blots   Charles M Griswold
• Ligation - dephosphorylation?   Duke Groebe
• Problem: aggregation of bact. expressed proteins.   Duke Groebe
• easy enzyme assay   Bob Gross
• easy enzyme assay   Bob Gross
• sequencing of PCR products   Barbara B Grossmann
• GST fusion protein   Xin Guo
• PCR to make small labeled probes   S. Gupta
• cloning bacterial DNA in Lambda   H3932Kis%HUELLA.earn at earn-relay.ac.uk
• chemiluminescent immunodetection   Peggy Halleck
• Stratagene Robocycler   Dan Handley
• Stratagene Robocycler   Dan Handley
• Automated Sequencing Cost?   Richard R. Hardy
• Contaminating plasmid   Dave Harry
• Contaminating plasmid   Dave Harry
• Home made gems don't "invert"   Dave Harry
• Proteinase K thawed...AOK??   Andrew Healey
• message to promega   Andrew Healey
• Yeast response to endotoxin?   Paul N Hengen
• Home made gems don't "invert"   Paul N Hengen
• Contaminating plasmid   Paul N Hengen
• xgal/iptg selection: summary   Paul N Hengen
• Pharmacia U.S.E. Mutagenesis   Paul N Hengen
• nash effect with phagemids   Paul N Hengen
• Large plaques in lambda libraries   Paul N Hengen
• PAGE - Silver Stain   Paul N Hengen
• optimizing CIP   Bernard Heymann
• GelManager-Biosystematica   Anthony Hilton
• S1 hydridization for determination of RNA levels   Heidi Lanora Hinkel
• DNA from tissue in formalin   Kathie Hodge
• IBI/Pustell sequence software   Rosemary Hoffman
• Home made gems don't "invert"   Rosemary Hoffman
• Home made gems don't "invert"   Robert Horton
• Home made gems don't "invert"   Robert Horton
• Some batches of light mineral oil can kill your PCR or RAPD reactions!   Robert Horton
• Mass spectroscopy of proteins   Eric R. Hugo
• 35S volatility and incubators (an old thread revisited)   James B. Hutchins
• Help, Actin   James B. Hutchins
• search for mecillinam, a beta-lactam antibiotic   James B. Hutchins
• How to stop plasmids losing inserts?????   IBR100E at ODUVM.BITNET
• Odd problem with sequencing gels   The doctor is IN.
• Thanks to all you helped me last query   The doctor is IN.
• pgEX-2t complete sequence help   Horst Ibelgaufts
• mammalian transfection hmw genomic DNA protocol   Horst Ibelgaufts
• Contaminating plasmid   Abiye Iyo
• Carrier molecules   JOHN
• ECL Quantitation of westerns   JOHNSON_T
• 50bp PCR Product   JOHNSON_T
• Re. Kanamycin resistant gene   JONG, SONG-MUH J
• Re. Is milk protein Y-phosphorylated?   JONG, SONG-MUH J
• Re. Bacterial strains for electroporation   JONG, SONG-MUH J
• Odd problem with sequencing gels   Dr. I.J. Jackson
• Obtaining Sac. Pombe   Mahesh V. Jayaraman
• polylysine interaction with DNA   Thomas R. Johnson
• Solubilising PAGE gels (probably a FAQ)   daj (David Johnston)
• PAGE - misformed wells - help   daj (David Johnston)
• phone # of Hybaid Ltd.   daj (David Johnston)
• sequencing gels; problems with plates and combs   daj (David Johnston)
• Message to anyone from Promega further info   daj (David Johnston)
• linearity of x-ray film/autorads   daj (David Johnston)
• Separation of immune complex   Song-Muh Jong
• Multiple repeated posting from mail   Song-Muh Jong
• test   Jose_Costoya
• Looking for GH4C1 cells   K.D.Preuss
• Xgal   R. A. KELLN
• Contaminating plasmid   R. A. KELLN
• Is there radioactive SDS available?   R. A. KELLN
• iec centrifuges   R. A. KELLN
• Lac repressor antibody   KRESS at ARTHUR.CITI2.FR
• Solubilising PAGE gels (probably a FAQ)   Ron Kagan
• Transforming LINEAR PLASMID . How ?   Ali Karami
• Optimizing PCR Reactions   Ali Karami
• Electroporation ( ? )   Ali Karami
• Xgal   Stephen Karp
• HPLC columns   Steve A. Kay
• Hydrophobic Chromatography   Steve A. Kay
• plasmid integration after transfection   Martin Kennedy
• PAGE - misformed wells - help   Rasheed Khalid
• What does A, C rich sequence mean?   Chong-Yee Khoo
• address needed   Byung-hoon Kim
• Thanks for address   Byung-hoon Kim
• Baekon 2000   Klaus.Matthaei at anu.edu.au
• protein stability at high conc.   Hannes Klump
• windows-program for constructing plasmids   Hannes Klump
• quantitative westerns   Hannes Klump
• Reporter gene vectors   Cornelius Krasel
• GST-fusion-protein   Cornelius Krasel
• sequencing gels; problems with plates and combs   Cornelius Krasel
• IMPORTANT - Mail and News Problems at net.bio.net   Dave Kristofferson
• UPDATE - Mail-To-News Problems at net.bio.net   David Kristofferson
• IMPORTANT BIOSCI INFORMATION   David Kristofferson
• IMPORTANT- PLEASE READ ASAP - SYSTEM PROGRESS REPORT   David Kristofferson
• transfection of HUVE cells?   L.Y.Chau
• Preparative IEF of proteins   LYNDON at BMS-UNIT.ICR.AC.UK
• PCR of Lambda Libraries   Dean Danner Lab
• phone # of Hybaid Ltd.   Dean Danner Lab
• Yeast ADE2 plasmid needed   Dean Danner Lab
• I vs GATC in PCR oligos   Dean Danner Lab
• PCR optimizatio   Parkinson Lab
• pUC118 sequence   Parkinson Lab
• PCR Multiplex   Robert Lagace
• tobacco acid pyrophosphotase   Morag Lancaster
• dsRNA transfer   Morag Lancaster
• Determining polymerase activity   Morag Lancaster
• Small plasmid DNAs   Joerg Langowski
• Tyrosine sulphate   Bob Lauder
• promoter analysis   Martin Leach
• Primer Extension Problem!   Martin Leach
• S1 hydridization for determination of RNA levels   Martin Leach
• Scientific clip art   Martin Leach
• Message to anyone from Promega   Martin Leach
• Message to anyone from Promega   Martin Leach
• Message to anyone from Promega further info   Martin Leach
• Minipreps/Promega   Martin Leach
• Real time science discussion   Martin Leach
• ***** REAL TIME SCIENCE DISCUSSION OVER THE NET *****   Martin Leach
• ### REAL TIME SCIENCE DISCUSSION ###   Martin Leach
• DNA isolation from gel (Re: Magic minipreps--DNA don't cut)   Jason Lei
• Electroporation   Dave Lennon
• Electroporation   M. William Lensch
• 50bp PCR Product   M. William Lensch
• SDS removal from samples   Walt Lilly
• A strain that provides unmethylated lambdaZAP DNA ?   Casper Linnestad
• A strain that provides unmethylated lambdaZAP DNA ? CORRECTION!!   Casper Linnestad
• Please show me the storage method of HL-60 cell line!.2   Joseph Lipsick
• Phosphorimager recommendation?   Dmitry Anatolievitch Los
• unsubscribe   Dmitry Anatolievitch Los
• cDNA kit recommendations SUMMERY   Michael Lush
• Iron ions in my DNA   Mr. M.J. Lush
• Xgen Corp. whereabouts   Leonard Lutter
• Electroporation into intact plant cells   MALBOOBI at QUCDN.QueensU.CA
• Using DNA markers to check size of RNA   BARRY J MARGULIES
• Preferential insert orientation   BARRY J MARGULIES
• Preferential insert orientation   BARRY J MARGULIES
• bandshift-PRT stuck in wells   MASSON
• what's the best probe to quantitate RNA in Northern blot????   SEPPO KAIJALAINEN MMETT
• Kanamycin resistance plasmids   MYGALP01 at ulkyvm.louisville.edu
• Xgal   MYGALP01 at ulkyvm.louisville.edu
• QuickHyb   MYGALP01 at ulkyvm.louisville.edu
• Gene-Clean double bands   Jose R. Macias
• Reagent packers gone amok?   Helgi Briem Magnusson
• test   Timothy Mahony
• CHEF gel problems   Rafa Maldonado
• Episome Transfer   Rafa Maldonado
• Magic minipreps--DNA don't cut   Rafa Maldonado
• Hard to dissolve eucaryotic DNA (Q)   Rafa Maldonado
• Plasmid DNA Purification   Rafa Maldonado
• PCR optimizatio   Rafa Maldonado
• Proteinase K thawed...AOK??   Rafa Maldonado
• PCR optimizatio   Rafa Maldonado
• triparental conjgation prblm   Rafa Maldonado
• How to stop plasmids losing inserts?????   Rafa Maldonado
• optimizing CIP   Rafa Maldonado
• Test   Rafael Maldonado
• what's the best probe to quantitate RNA in Northern blot????   Michael Man
• Phosphorimager recommendation?   Jonathan B. Marder
• linearity of x-ray film/autorads   Jonathan B. Marder
• Phosphorimager   Mark
• Preferential insert orientation   Mark
• repost: cellulase-minus E. coli   Mary Metzler, Plants, x8076
• m.blue staining of DNA   Marianna Max
• HELP! unusual cell growth   Marianna Max
• DNA heterozygosity   John H McDonald
• Immunolocalization of fish calcitonin   Phil McFadden
• MMLV RT Protocol Request   Daniel Meier
• request for embl e-mail address   Pierre-Alain Menoud
• cells in the middle do not express the protein   Eric H. Mercer
• Pichia/Hansenula   Christian Meyer
• linearity of x-ray film/autorads   David J. Meyer
• IEF of a small protein   Ding Ming
• Mass spec services?   Ding Ming
• gc's post about a strange band on page   Ding Ming
• PAGE - misformed wells - help   Ding Ming
• problem with ptn squcing   Shahram Mori
• triparental conjgation prblm   Shahram Mori
• HELP problem with lambda   Mr. G. Morley
• finding the right phosphorimager   Mike Morris
• Your thoughts on Hot PCR for microsatellite studies   Mike Morris
• Air Thermo Cycler ????   Tom Morrison
• CsCl-EtBr purification   Peter M. Muriana
• Xgal   Peter M. Muriana
• Xgal   Bernard Murray
• digitonin in nuclei isolation buffer - why?   Bernard Murray
• Iron ions in my DNA   Bernard Murray
• SDS removal from samples   Bernard Murray
• x-linking   Christopher A Myers
• DNA from tissue in formalin   Ola Myklebost
• Effects of Argon   Ramin Charles Nakisa
• sequencing of PCR products   John Nash
• spotty blots   John Nash
• sequencing gels; problems with plates and combs   John Nash
• ELECTROPORATION of E. coli   John Nash
• nash effect with phagemids   John Nash
• RT-PCR from few cells.   Richard Near
• digitonin in nuclei isolation buffer - why?   Don Newmeyer
• expression of membrane proteins in pichia   Don Newmeyer
• Nonradioactive labeling(Peroxidase or AP..)   I-shiou Ng
• BIOS Corporation Address needed   Chia Jin Ngee
• promoter analysis   Soeren Jensby Nielsen
• Alu sequence   Soeren Jensby Nielsen
• electroporation of LCLs   Thierry Nouspikel
• test   Dimitri Odorico
• Your thoughts on Hot PCR for microsatellite studies   Dimitri Odorico
• pSelect/pAlter-1 seq needed   Francis Ouellette
• DNA: HB101-> C600   Jim Owens
• salvaging freezer contents   Jim Owens
• Home made gems don't "invert"   Jim Owens
• Hard to dissolve eucaryotic DNA (Q)   Jim Owens
• MD Personal Densitometer   RICHARD P. PHIPPS
• Interpolation methods   JOHN PROUDMAN
• MAb purification   JOHN PROUDMAN
• Preparative IEF   JOHN PROUDMAN
• IEF   JOHN PROUDMAN
• detection system for cloning chitinase gene   Chanpen Wiwat - PYMI
• Electroanalitical methods   Mauricio Cesar Palmieri
• Electroanalitical methods   Mauricio Cesar Palmieri
• Site-directed mutagensis without subcloning   Julian Parkhill
• Inducing SOS response   Christopher Coward Path
• Using Labview from National Instruments in Mol. Biol.   Jean Peccoud
• sequencing gels; problems with plates and combs   Anthony J. Persechini
• sequencing gels; problems with plates and combs   Anthony J. Persechini
• Mg and PCR   Petur Henry Petersen
• Optimizing PCR Reactions   Petur Henry Petersen
• Optimizing PCR reactions   Petur Henry Petersen
• Contaminating plasmid   James N Petitte
• Air Thermo Cycler ????   James N Petitte
• Heat stable E.coli protein   Dr. Barry Phipps
• Fusion vector required!   Mr D J Plows
• iec centrifuges   Brent Pollock
• Radiolabelled ANS (1-anilinonapthalene-8-sulfonic acid) or Bis-ANS   Peter Prevelige
• BuildSampleSheet for ABI sequencer   Jaime Prilusky
• what's the best probe to quantitate RNA in Northern blot????   Przemko
• Reporter gene vectors   Przemko
• Northern blot with ribosomal RNA bands   Przemko
• remove his-tag   QIONG at UNCVX1.OIT.UNC.EDU
• Who sells the Tropi-Cooler water bath?   RTWORRELL at eaton.physio.emory.edu
• Purifying Chicken IgY   RYBICKI, ED
• Protein/DNA isolation   RYBICKI, ED
• Magic minipreps--DNA don't cut   RYBICKI, ED
• Minipreps/Promega   RYBICKI, ED
• spotty blots   RYBICKI, ED
• Preparative IEF of proteins   Uttam Rajbhandary
• DNA conc by EtBr staining?   Randy P. Rasmussen
• Making a TA Cloning Vector   Randy P. Rasmussen
• linearity of x-ray film/autorads   Paul Repacholi
• Immunoprecipitation of INTACT vesicles   Randall K. Ribaudo
• Plasmid DNA Purification   James F Rice
• HELP! unusual cell growth   Robert Kuhelj, IJS
• spotty blots   Wendy Robinson
• DNA from tissue in formalin   Wendy Robinson
• optimizing CIP   Steve Rodems
• 50bp PCR Product   Steve Rodems
• PCR to make small labeled probes   Steve Rodems
• pUC118 sequence   Paul Roessler
• Phage Display Vectors   Paul Romaniuk
• Fusion vector required!   Frederick Roth
• Small c:a 50bp PCR-products, visualization?   RoundUp
• Trouble growing EMBL4 to high titer   Robert Roxby
• test   Mike Rund
• Yeast ADE2 plasmid needed   Peter J. Russell
• mac-densitometer   DAVID SCOTT
• linearity of x-ray film/autorads   DAVID SCOTT
• thermal cyclers (MJ and Hybaid)   DAVID SCOTT
• iec centrifuges   DAVID SCOTT
• ThankYOU   DAVID SCOTT
• Biomedical Technol Address wanted   ARMIN SEPP
• Epitope tags and Antibody sources   Roland J Saldanha
• Nonspecific riboprobe detection of 28S Ribosomal RNA?   David M. Sander
• decarboxylation help   C R Santhosh-Kumar
• Pharmacia U.S.E. Mutagenesis   Sharon Schendel
• salvaging freezer contents   Jacquie Schwartz
• HEPG2 Aldehyde Dehydrogenase Assay   Mark Schweder
• SDS removal from samples   Mark Schweder
• Northern blot with ribosomal RNA bands   Scott A Selby-1
• Help: stripping northern blots   Jorge L. Sepulveda
• DNA ppt w NaCl   Basavaraju Shankarappa
• Sequencing a hairpin?   Shauna R. Brummet, Ph.d.
• Direct sequencing of PCR products   Shauna R. Brummet, Ph.d.
• Please show me the storage method of HL-60 cell line!.2   Yoshiyuki Shibata
• Electroporation   Yoshiyuki Shibata
• Thanks: Electroporation   Yoshiyuki Shibata
• Thanks: storage method   Yoshiyuki Shibata
• cell sorting/analysis in the bay area ?   Dov Shiffman
• Desperately Seeking Coli   Nathan O. Siemers
• Blocking agents for DIG labelling   Andrew Simmonds
• DNA anyone ?   Jim Sloan
• western for membrane protein   Tyrone Slothrop
• pGEX with NdeI site-help   Blake Smith
• Carrier molecules   Mark Solomon
• Streptothricin/Nourseothricin source?   A.E. Souza
• problem synthesizing full-length riboprobe   Stephan Spencer
• Kanamycin resistance plasmids   Jamie Stanek
• Nutrient medium for cell culture   Oh Kah Weng Steve
• Nutrient medium for cell culture ..cont.   Oh Kah Weng Steve
• Nutrient medium for cell culture ..cont.   Oh Kah Weng Steve
• Protein A for surface IgG alignment ?   Ed Stokes
• PAGE - misformed wells - help   Gregory Stuart
• sequencing of PCR products   Kathy Stupka
• Some batches of light mineral oil can kill your PCR or RAPD reactions!   James S. Sutcliffe
• Gel documentation systems   George Thomas
• media & promega minipreps   Thomas_C.Newman
• sequencing gels; problems with plates and combs   Graham Threadgill
• sequencing gels; problems with plates and combs   Graham Threadgill
• Ligation - dephosphorylation?   KLADE Torsten
• Site-directed mutagensis without subcloning   Michael J. Tuvin
• Xgal   Chris Upton
• Xgal   Chris Upton
• ELECTROPORATION of E. coli   Chris Upton
• PC program for drawing plasmid maps   VOSS at AIMP1.UNA.AC.AT
• uncoding programmes from ftp sites   Hugo Volkaert
• SUGGESTION ABOUT BUYING A ELECTROPORATION INSTRUMENT   WANGNN at UCBEH.SAN.UC.EDU
• nash effect and phagemids   Warren Wakarchuk
• ALF Automated sequencer   Matthew Wakefield
• Northern blot with ribosomal RNA bands   Matthew Wakefield
• RNA size fractionation   Matthew Wakefield
• DNA: HB101-> C600   FJ van der Wal
• Solubilising PAGE gels (probably a FAQ)   R.G. Walters
• neurosecretory protein isolation   Chiou-Miin Wang
• help: plant nucleus prep   Tao Wei
• help-how to access GCG   Tao Wei
• GST fusion prot isolation   Elaine M. Weidenhammer
• transposon Tn916   C v/d Weijden
• TABLE and SILMUT Programs   Helge Weissig
• what's the best probe to quantitate RNA in Northern blot????   Helge Weissig
• salvaging freezer contents   Helge Weissig
• Very low pH buffer   Helge Weissig
• Earthquake Infos   Helge Weissig
• alkaline phosphatase antibodies   Helge Weissig
• ABI automated sequencing using T7   Helge Weissig
• Subcloning with oligos   Helge Weissig
• large phages/clonetech reply/packaging extracts   Helge Weissig
• Making a TA Cloning Vector   Helge Weissig
• Construction of TA cloning vector   Helge Weissig
• optimizing CIP   Helge Weissig
• Gel documentation systems   Ross Whetten
• Contaminating plasmid   Sheryl White
• Magic minipreps--DNA don't cut   Sheryl White
• Optimizing PCR Reactions   Sheryl White
• Double Take   Roger Wiegand
• data capture software   Janet Williams
• Which sequence analysis package?   Jim Woodgett
• Which sequence analysis package?   Jim Woodgett
• Efficiency of bacterial tx with linear DNA?   Jim Woodgett
• Is milk protein Y-phosphorylated?   Jim Woodgett
• Bisbenzimide extraction   Zhongtang Yu
• DNA extraction from BAC   Zhongtang Yu
• Double Take   ZURLINDEN at IMB.unibe.ch
• Genius system & heterologous hybs?   Tracey Zacharias
• Plant DNA Isolation and Restriction   Tracey Zacharias
• DNA ppt'n with 4M NaCl   Tracey Zacharias
• DNA stuck in wells in gel retardation assay   Xin Zhang
• Summary of DNA-Protein aggregation in gel binding assay   Xin Zhang
• Site-directed mutagensis without subcloning   Wei Zhou
• Northern blot with ribosomal RNA bands   Wei Zhou
• CsCl-EtBr purification   Frances Hannan Zoology
• Which sequence analysis package?   Frances Hannan Zoology
• salvaging freezer contents   Frances Hannan Zoology
• lambda packaging contamination   Frances Hannan Zoology
• lambda packaging contamination   Frances Hannan Zoology
• DNA from tissue in formalin   aforus
• Contaminating plasmid   ashendel at aclcb.purdue.edu
• Human Small Intestinal Cell Line   bahr at celsus.oci.utoronto.ca
• FW: Returned Mail   bates
• Subcloning with oligos   bates
• K Ferricyanide   bates
• RACE-PCR Kits   bates
• Change of email address   bates
• Bacterial Genetics   daniel berman
• electroporation of LCLs   brett at borcim.wustl.edu
• iron ions   brett at borcim.wustl.edu
• vaccinia   brett at borcim.wustl.edu
• What does A, C rich sequence mean?   chai_z at wehi.edu.au
• Magic minipreps--DNA don't cut   choie01 at mcclb0.med.nyu.edu
• PCR Restriction Site engineering   choie01 at mcclb0.med.nyu.edu
• spotty blots   dLee
• Is milk protein Y-phosphorylated?   dLee
• Bisbenzimide extraction   dallas at mcvax4.d48.lilly.com
• Episome Transfer(soft music,low lights)   dallas at mcvax4.d48.lilly.com
• Multiple DNA sequence alignment   danismit
• Nonspecific riboprobe detection of 28S Ribosomal RNA?   denton at usuhs.ucc.usuhs.nnmc.navy.mil
• Help: stripping northern blots   denton at usuhsb.usuhs.mil
• Ericomp thermal cyclers   dkatz at earth.doane.edu
• DNA from Biopsy   dkim at unm.edu
• Odd problem with sequencing gels   dkim at unm.edu
• Small c: a 50bp PCR-products, visualization?   dspete at helix.nih.gov
• Inducing SOS response   e_hugo at dsu1.dsu.nodak.edu
• schematics for thermal cyclers   e_hugo at dsu1.dsu.nodak.edu
• Coupling DNA to Sephadex?   edbeaty
• Fidelity of Thermostable DNA Polymerases   erfi at eel.sunet.se
• Protein/DNA isolation   ez002976 at othello.ucdavis.edu
• Protein/DNA isolation   ez002976 at othello.ucdavis.edu
• PCR optimizatio   douglas l feinstein
• PCR optimizatio   douglas l feinstein
• thermal cyclers   douglas l feinstein
• PCR and glycogen   douglas l feinstein
• phone # of Hybaid Ltd.   douglas l feinstein
• screening across species   douglas l feinstein
• Subcloning with oligos   douglas l feinstein
• screening across species   douglas l feinstein
• thermal cyclers (MJ and Hybaid)   douglas l feinstein
• Alk Phos conjugated oligos   douglas l feinstein
• rRNA and polytranscripts????   forrest
• 'POSSUM'PRINTING   frances at matai.vuw.ac.nz
• Primer Extension Problem!   frank at omrf.uokhsc.edu
• easy enzyme assay   fwhyde at mmm.com
• Plasmid DNA Purification   fwhyde at mmm.com
• MAb purification   fwhyde at mmm.com
• Northern blot with ribosomal RNA bands   fzestabr at chip.ucdavis.edu
• DNA stuck in wells in gel retardation assay   gc
• optimizing CIP   gc
• optimizing CIP   gc
• BACILLUS SUBTILIS STRAINS NEEDED   gurjit at imtech.ernet.in
• Help: stripping northern blots   h-seifert at nwu.edu
• Northern blot with ribosomal RNA bands   h-seifert at nwu.edu
• subscription (Methods-and reagents)   h9390031 at HKUSUB.HKU.HK
• iron ions in dna   john hachey
• DNA ppt w NaCl   john hachey
• Is milk protein Y-phosphorylated?   paul haney
• E-mail Address Request   paul haney
• NASBA   hauge at Frodo.MGH.Harvard.EDU
• sequencing service   Domingos henrique
• Announcement: New program for designing degenerate oligos   ramin homayouni
• cDNA kits   jac227 at huxley.anu.edu.au
• Gel fixing   jenkins at aidsun.nibsc.ac.uk
• q. on tiling programs   jmv at CANCTR.MC.DUKE.EDU
• sequencing of PCR products   jmv at CANCTR.MC.DUKE.EDU
• 840AV and image analysis   jurgenson at cobra.uni.edu
• Where did everybody go?   ktgeiss at miavx1.acs.muohio.edu
• Subscription info needed...   ktgeiss at miavx1.acs.muohio.edu
• A sleazy way to avoid direct PCR Sequencing   stuart kuhstoss
• Which sequence analysis package?   tzu-ping lin
• what's the best probe to quantitate RNA in Northern blot????   tzu-ping lin
• Protein A for surface IgG alignment ?   littgj at wmvx.lvs.dupont.com
• Phosphorimager recommendation?   lsalati at wvnvms.wvnet.edu
• CHEF gel problems   rick lynch
• Help:RT assay wanted.   macdouga at wren.sunnybrook.utoronto.ca
• 840AV and image analysis   carol macleod
• pRSVCATX   mcgraw at bscr.uga.edu
• Carbocyanine dye DiOC5(3)   mcveylab at vetmail.trotnet.vet.ksu.edu
• Cellulase minus E. coli strains   metzler at sara.cc.utu.fi
• genomic phage libraries   metzler at sara.cc.utu.fi
• promoter analysis   mhollowa at epo.som.sunysb.edu
• Promoter analysis   mhollowa at epo.som.sunysb.edu
• Plasmid Prep for Sequencing (Sorry if this is FAQ)   mhollowa at epo.som.sunysb.edu
• ABI automated sequencing using T7   mic255z at vaxc.cc.monash.edu.au
• TRISOLV RNA extraction from Gram +ves   mic268a at vaxc.cc.monash.edu.au
• Quantitative RT-PCR   michael at uwovax.uwo.ca
• Help needed to clone a gene   mingfeng at kean.ucs.mun.ca
• digitonin in nuclei isolation buffer - why?   mmelan at pattie.wellesley.edu
• Magic minipreps--DNA don't cut   mrege at samba.cnb.uam.es
• <None>   mrege at samba.cnb.uam.es
• Magic minipreps--DNA don't cut SUMMARY   mrege at samba.cnb.uam.es
• <None>   mrege at samba.cnb.uam.es
• Magic minipreps--DNA don't cut SUMMARY   mrege at samba.cnb.uam.es
• Immuno-PCR   nika
• Immuno-PCR   nika
• 35S volatility and incubators (an old thread revisited)   nishir at ohsu.edu
• Kanamycin resistance plasmids   nishir at ohsu.edu
• Some batches of light mineral oil can kill your PCR or RAPD reactions!   nishir at ohsu.edu
• How to stop plasmids losing inserts?????   nishir at ohsu.edu
• xgal/iptg selection: summary   osp086 at vaxa.bangor.ac.uk
• PCR and glycogen   osp086 at vaxa.bangor.ac.uk
• coycycler   pcrossen at chmeds.ac.nz
• Thermal cyclers   pcrossen at chmeds.ac.nz
• TEST   carlos h. pedemonte
• more about X-gal   carlos h. pedemonte
• expression of membrane proteins in pichia   carlos h. pedemonte
• Air Thermo Cycler ????   carlos h. pedemonte
• Is there radioactive SDS available?   carlos h. pedemonte
• cells in the middle do not express the protein   carlos h. pedemonte
• How to couple IgGs to CL-4B ???   pesusi at sara.cc.utu.fi
• western for membrane protein   pesusi at sara.cc.utu.fi
• Message to anyone from Promega   pmiguel at bilbo.bio.purdue.edu
• Questions regarding RNA purification from plants   q00023 at TIGGER.STCLOUD.MSUS.EDU
• unsubscribe   ratner_l at MSdisk.WUstl.EDU
• Reporter gene vectors   riyer at rascal.med.harvard.edu
• Genius system & heterologous hybs?   rx81238 at mcvax4.d48.lilly.com
• cDNA library (lambda MOSSLOX)   sarven
• Mitochondrial DNA isolation   sarven
• Signal Seq. Trap   schren13 at uctvax.uct.ac.za
• plasmid integration after transfection   suter at VAX.mpiz-koeln.mpg.d400.de
• help problem with lambda   suter at VAX.mpiz-koeln.mpg.d400.de
• alu sequence   suter at VAX.mpiz-koeln.mpg.d400.de
• mammalian transfection/integration   suter at VAX.mpiz-koeln.mpg.d400.de
• protein expression   suter at VAX.mpiz-koeln.mpg.d400.de
• message to promega   suter at VAX.mpiz-koeln.mpg.d400.de
• spotty blots   suter at VAX.mpiz-koeln.mpg.d400.de
• nash effect with phagemids   suter at VAX.mpiz-koeln.mpg.d400.de
• the nash effect/phagemid or pUC ?   suter at VAX.mpiz-koeln.mpg.d400.de
• <None>   swift at mcvax2.d48.lilly.com
• Hard to dissolve eucaryotic DNA (Q)   swift at mcvax2.d48.lilly.com
• BIOS Corporation Address needed   swift at mcvax4.d48.lilly.com
• Fusion vector required!   szdbates at chip.ucdavis.edu
• phone # of Hybaid Ltd.   szdbates at chip.ucdavis.edu
• optimizing CIP   tronni at ktl.fi
• E. coli strains for electroporation   txpljfg at UABCVSR.cvsr.uab.edu
• PC program for drawing plasmid maps   txpljfg at UABCVSR.cvsr.uab.edu
• Small c:a 50bp PCR-products, visualization?   txpljfg at UABCVSR.cvsr.uab.edu
• PC program for drawing plasmid maps   txpljfg at UABCVSR.cvsr.uab.edu
• ***** REAL TIME SCIENCE DISCUSSION OVER THE NET *****   txpljfg at UABCVSR.cvsr.uab.edu
• pML-neo info needed   unknown at dl.ac.uk
• NASBA   unknown at dl.ac.uk
• Which sequence analysis package?   unknown at dl.ac.uk
• CPK molecular models purchasing onfo needed   unknown at dl.ac.uk
• Hi   unknown at dl.ac.uk
• Chicken IgY purification   unknown at dl.ac.uk
• salvaging freezer contents   unknown at dl.ac.uk
• Mg & extraneous bands   unknown at dl.ac.uk
• Mg and bands (cont.)   unknown at dl.ac.uk
• finding the right phosphorimager   unknown at dl.ac.uk
• Contaminating plasmid   unknown at dl.ac.uk
• pSelect/pAlter-1 seq needed   unknown at dl.ac.uk
• B-gal in LCLs   unknown at dl.ac.uk
• Optimizing PCR Reactions   unknown at dl.ac.uk
• transformation with denatured plasmids   unknown at dl.ac.uk
• DNA transfer check   unknown at dl.ac.uk
• re transfer check   unknown at dl.ac.uk
• Restriction in Gels   unknown at dl.ac.uk
• phone # of Hybaid Ltd.   unknown at dl.ac.uk
• Please tell me the storage method of HL-60 cell line!   unknown at dl.ac.uk
• Optimizing PCR reactions   unknown at dl.ac.uk
• windows-program for constructing plasmids   unknown at dl.ac.uk
• Gene Runner program !   unknown at dl.ac.uk
• Minipreps/Promega   unknown at dl.ac.uk
• xgal/iptg selection: summary   unknown at dl.ac.uk
• protein expression   unknown at dl.ac.uk
• MAb purification   unknown at dl.ac.uk
• thermal cyclers   unknown at dl.ac.uk
• transgenic rats   unknown at dl.ac.uk
• thermal cyclers   unknown at dl.ac.uk
• Odd problem with sequencing gels   unknown at dl.ac.uk
• what's the best probe to quantitate RNA in Northern blot????   vangeldr at cmgm.stanford.edu
• Magic minipreps--DNA don't cut   wetsel_r at wums.wustl.edu
• HL-60 cells   wetsel_r at wums.wustl.edu
• Message to anyone from Promega   wetsel_r at wums.wustl.edu
• Pharmacia U.S.E. Mutagenesis   wetsel_r at wums.wustl.edu
• Strains to select for Sup F containing plasmids   ww40
• CIP damages overhangs?   ww40
• help needed purification asolectin from fluorescence   zeljkos at vms.ucc.okstate.edu
• help purifying asolectin from fluorescent impurities   zeljkos at vms.ucc.okstate.edu

Last message date: Mon Jan 31 23:13:46 EST 1994
Archived on: Sun Mar 6 20:04:04 EST 2005

• Messages sorted by: [ thread ] [ subject ] [ date ]
• More info on this list...