Lauri Lintott (llintott at ACS.UCALGARY.CA) wrote:
: You can not use TBE buffer to run gels which you want to purify
: bands from. Try a buffer such as TAE.
: 50 X TAE
: 2M Tris base
: 0.05 M EDTA (pH 8.0)
: 57.1 ml Glacial Acetic Acid (per L)
: Lauri Lintott
: Bio. Sci. U of Calgary
But surely you can! I think the people were talking about geneclean II
that includes (as well as the USB Kit) a TBE Modifier - and both kits
work really great with agarose in TBE buffer !!!!
[ Sebastian.Bunka at vu-wien.ac.at ]
Location: earth, europe, austria, vienna Inst. of Bacteriology Vet.Univ.