Large-scale monocyte isolation

JOHNSON, MICHAEL JOHNSON+c_MICHAEL%A1%Mallinckrodt at mcimail.com
Fri Jul 1 09:28:34 EST 1994


Netters,
I am trying to study the interaction of several porcine cell types. I am 
attempting to isolate primary cultures of several cell lines from the same 
animal. To do this work I need to isolate monocytes from a large amount (@ 2 l) 
of porcine blood (so that I can freeze back as many cells as possible). I would 
like to know if anyone is doing work of this nature  and what method would be 
the most efficient. Also, what medium is required for culturing these cells?

Thanks,
Michael Johnson

johnson+c_michael%a1%mallinckrodt at mcimail.com



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