I am trying to study the interaction of several porcine cell types. I am
attempting to isolate primary cultures of several cell lines from the same
animal. To do this work I need to isolate monocytes from a large amount (@ 2 l)
of porcine blood (so that I can freeze back as many cells as possible). I would
like to know if anyone is doing work of this nature and what method would be
the most efficient. Also, what medium is required for culturing these cells?
johnson+c_michael%a1%mallinckrodt at mcimail.com