unmodified T7 DNA polymerase

STRUCTURAL NEUROBIOLOGY XTAL LAB- MADE FROM 100% RECYCLED ELECTRONS GLUXLAB at MSVAX.MSSM.EDU
Fri Jul 1 08:37:33 EST 1994


Dear M and Rer's

	I am attempting to do the 
dut ung mutagenesis technique in Bluescript (2.9 Kb)
with a 2.2 Kb insert. T4 DNA pol and gene 32 protein
does not seem to do the job of making full length 
copies. I wanted to switch to T7 DNA pol. which
if you believe the hype, is better.
	BIO RAD is bloody expensive and Sequenase
is modified T7 which does NOT work.

	Any experiences and a SOURCE for
unmodified T7.

	Thanks, we are desperate.

Emer Ferro, PhD





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