unmodified T7 DNA polymerase
STRUCTURAL NEUROBIOLOGY XTAL LAB- MADE FROM 100% RECYCLED ELECTRONS
GLUXLAB at MSVAX.MSSM.EDU
Fri Jul 1 08:37:33 EST 1994
Dear M and Rer's
I am attempting to do the
dut ung mutagenesis technique in Bluescript (2.9 Kb)
with a 2.2 Kb insert. T4 DNA pol and gene 32 protein
does not seem to do the job of making full length
copies. I wanted to switch to T7 DNA pol. which
if you believe the hype, is better.
BIO RAD is bloody expensive and Sequenase
is modified T7 which does NOT work.
Any experiences and a SOURCE for
Thanks, we are desperate.
Emer Ferro, PhD
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