In Article <2uq4g1$svr at agate.berkeley.edu>,
lab_winoto at maillink.berkeley.edu. (Winoto lab) wrote:
>In article <CrE9Gu.Cx7 at acsu.buffalo.edu>
>vp23qw83 at ubvms.cc.buffalo.edu (Xin Guo) writes:
>>> Hi, Bionetters:
>>>> I have a trouble to get a high yield of PCR products by using Promega's
>> Wizard Prep method.....(stuff deleted)...
> I've routinely used the lithium chloride method of DNA isolation from lowmelt
>agarose and its worked quite well.....(more stuff deleted)....
Even better, try the NA45 membranes (DEAE) from S&S. These membranes are
great for recovering DNA from gels,and you don't even need to excise and
melt the band. Simply make a slit in front of the band, place a small
membrane strip into the slit, and electrophorese the DNA onto the membrane.
Then you wash the membrane and elute with a high-salt buffer, then
precipitate. The whole process takes about five or ten minutes. This is a
quantitative method, and you can even monitor recovery with a UV lamp in
case you screw it up. After years of using every other method under the sun,
I swear by this technique. Good luck!
Tracy Aquilla, Post-doctoral Research Associate
Department of Molecular Physiology and Biophysics
University of Vermont, College of Medicine
aquilla at salus.med.uvm.edu