Quantitating Radioactive label in DNA and RNA

Tracy Aquilla aquilla at salus.med.uvm.edu
Fri Jul 1 18:09:36 EST 1994


In Article <2us9ns$eso at matt.ksu.ksu.edu>, hlhinkel at ksu.ksu.edu (Heidi Lanora
Hinkel) wrote:
>        I am looking for methods with which to quanitate the
>amount of P32 -ATP incorperated by end-labeling with T4 kinase,
>in both DNA and RNA probes. I would like to hear of any methods
>that you know of and which one you think is best. Thanks in
>advance
>                Heidi Hinkel
>                hlhinkel at matt.ksu.ksu.edu
>                Division of Biology
>                Kansas State University
>                Manhattan, KS 66506
>
>
 I usually just count a small fraction of the probe after purification,
using a scintillation counter. How much more quantitative do you need to get?
Tracy



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