Quantitating Radioactive label in DNA and RNA
Tracy Aquilla
aquilla at salus.med.uvm.edu
Fri Jul 1 18:09:36 EST 1994
In Article <2us9ns$eso at matt.ksu.ksu.edu>, hlhinkel at ksu.ksu.edu (Heidi Lanora
Hinkel) wrote:
> I am looking for methods with which to quanitate the
>amount of P32 -ATP incorperated by end-labeling with T4 kinase,
>in both DNA and RNA probes. I would like to hear of any methods
>that you know of and which one you think is best. Thanks in
>advance
> Heidi Hinkel
> hlhinkel at matt.ksu.ksu.edu
> Division of Biology
> Kansas State University
> Manhattan, KS 66506
>
>
I usually just count a small fraction of the probe after purification,
using a scintillation counter. How much more quantitative do you need to get?
Tracy
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