IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

unmodified T7 DNA polymerase

Winoto lab lab_winoto at maillink.berkeley.edu.
Fri Jul 1 23:28:48 EST 1994


In article <01HE6R0WHKUA00115P at msvax.mssm.edu>
GLUXLAB at MSVAX.MSSM.EDU (STRUCTURAL NEUROBIOLOGY XTAL LAB- MADE FROM
100% RECYCLED ELECTRONS) writes:

>         I am attempting to do the 
> dut ung mutagenesis technique in Bluescript (2.9 Kb)
> with a 2.2 Kb insert. T4 DNA pol and gene 32 protein
> does not seem to do the job of making full length 
> copies. I wanted to switch to T7 DNA pol. which
> if you believe the hype, is better.
>         BIO RAD is bloody expensive and Sequenase
> is modified T7 which does NOT work.

Hi Emer,

Have you checked-out the New England Biolabs Catalog?  T7 DNA pol is
available (unmodified) in 200 or 1000 unit quantities ($50 and $200). 
Page 64 item #s 256S (200 units) or 256L (1000 units).

Good luck,

Paul
> 
>         Any experiences and a SOURCE for
> unmodified T7.



More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net