In article <01HE6R0WHKUA00115P at msvax.mssm.edu>
GLUXLAB at MSVAX.MSSM.EDU (STRUCTURAL NEUROBIOLOGY XTAL LAB- MADE FROM
100% RECYCLED ELECTRONS) writes:
> I am attempting to do the
> dut ung mutagenesis technique in Bluescript (2.9 Kb)
> with a 2.2 Kb insert. T4 DNA pol and gene 32 protein
> does not seem to do the job of making full length
> copies. I wanted to switch to T7 DNA pol. which
> if you believe the hype, is better.
> BIO RAD is bloody expensive and Sequenase
> is modified T7 which does NOT work.
Have you checked-out the New England Biolabs Catalog? T7 DNA pol is
available (unmodified) in 200 or 1000 unit quantities ($50 and $200).
Page 64 item #s 256S (200 units) or 256L (1000 units).
>> Any experiences and a SOURCE for
> unmodified T7.