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analysis of oligos help?

Robert Preston rapr at MED.PITT.EDU
Sat Jul 2 07:23:45 EST 1994


Hi netfolks, I'm wondering what is the easiest way to analyze oligos
(say, 15 - 40mers) for purity/sequence.  For example, has anyone done
SSCP on degenerate oligos and found the right number of bands?  If not,
why not.  Also, does HPLC resolve degenerate oligos into the components?
If so, what kind of column/apparatus is best for that purpose? 

Thanks for all input, I'll summarize and post the response.

Rob Preston
rapr at med.pitt.edu





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