According to the current protocols in Molecular Biology, the diffferential
display of 6% denaturing PAGE/Urea gel is dried at room temperature for
about 1 hour. Is that any reason the gel can not be dried with heat?
I have tried to dry it at RT for 1 hr and overnight. For 1 hr, the gel wasn'
t dried at all. For O/N drying, the gel was broken into pieces.
These 2 gels were dried without vacuum. One publication said the gel was
dried on the plate or transfered to a filter before exposed to X-ray film.
Do these people dry the gel without vacuum?
Any suggestion would be very much appreciated.