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How to dry the DDRT-PCR gel?

Hung-Yi Su suhy at kahu.lincoln.ac.nz
Sun Jul 3 23:07:32 EST 1994


Dear Netters:

According to the current protocols in Molecular Biology, the diffferential 
display of 6% denaturing PAGE/Urea gel is dried at room temperature for 
about 1 hour.  Is that any reason the gel can not be dried with heat?  
I have tried to dry it at RT for 1 hr and overnight.  For 1 hr, the gel wasn'
t dried at all.  For O/N drying, the gel was broken into pieces.   
These 2 gels were dried without vacuum.  One publication said the gel was 
dried on the plate or transfered to a filter before exposed to X-ray film.  
Do these people dry the gel without vacuum?

Any suggestion would be very much appreciated.


Hung-Yi



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