In article <2v8hif$j90 at mserv1.dl.ac.uk> ANDY PHILLIPS
<andy.phillips at afrc.ac.uk> (Tel +44 275 392181 EXT 257) writes:
>We have two plant cDNAs of unknown function that we want to express in E.
>coli for making antibodies. The expression vector should have as many as
>possible of the following features:
>1. T7 promoter
>2. C-terminal purification tag, eg 6xHis, to avoid isolation of truncated
>3. N-terminal tag for further purification eg GST
>4. Kan resistance, if possible, for plasmid stability (works better than Amp in
> our hands)
>>Anyone know where I could get hold of such a beast?
>andy.phillips at bbsrc.ac.uk>Long Ashton Research Station
>Bristol, BS18 9AF, UK
I don't think you're going to be able to buy such a vector from one
Novagen has the sole distribution rights to the Studier pET vector system based
on the T7 promoter and a couple of these pET vector provide a C-terminal 6xHis
tag. I believe that the pET24 series has C-terminal 6xHis tag and uses
Pharmacia seems to have the distribution rights to the GST vectors (pGEX).
Other company may offer non-Studier type T7 promoter based expression vectors,
so it's still possible that some one out there sells just what you want. I
have my doubts. But you could create your ideal vector from the Novagen pET
and the Pharmacia pGEX.
I suspect, though, that either pET or pGEX alone will be sufficient for your
needs (overexpressing polypeptides for antibodies).
Oh, Novagen's distributor in Europe is AMS Biotechnology (UK tel:
44-0-993-706500, UK fax: 44-0-993-706006). And Pharmacia, is, well,
Hope this helps. Usual disclaimers apply: no connection to Novagen or
Pharmacia except using their products, etc..
Institute for Molecular Biology and Biophysics
ETH-Honggerberg (Swiss Federal Institute of Technology)
8093 Zurich, Switzerland
email: tan at aeolus.vmsmail.ethz.ch