Who sells my ideal E. coli expression vector?
andy.phillips at afrc.ac.uk
Mon Jul 4 03:34:23 EST 1994
We have two plant cDNAs of unknown function that we want to express in E.
coli for making antibodies. The expression vector should have as many as
possible of the following features:
1. T7 promoter
2. C-terminal purification tag, eg 6xHis, to avoid isolation of truncated
3. N-terminal tag for further purification eg GST
4. Kan resistance, if possible, for plasmid stability (works better than Amp in
Anyone know where I could get hold of such a beast?
andy.phillips at bbsrc.ac.uk
Long Ashton Research Station
Bristol, BS18 9AF, UK
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