TA cloning vectors

berschic at convex.rz.uni-duesseldorf.de berschic at convex.rz.uni-duesseldorf.de
Mon Jul 4 06:41:20 EST 1994


In article <199407022309.QAA24423 at net.bio.net> hwyckoff at ASRR.ARSUSDA.GOV ("HERBERT WYCKOFF") writes:
>
>In the not too distant past,  I seem to remember a posting of a method
>for producing "TA" vectors by modifying blunt cut vectors with Taq
>polymerase.  If someone would please send me a copy of the protocol,
>I would appreciate it.
>
>Thanks
>
>Herb Wyckoff
>HWYCKOFF at ASRR.ARSUSDA.GOV
>
>
Hi Herb,
I read something of tailing a blunt end vector with a ddTTp. 
That adds only one nucleotide and this vector could be used 
for cloning PCR products. So a normal tailing protocol with 
ddTTP instead of dTTP should work.
Peter



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