transducing recA w/o tetR ?
mbrgw at s-crim1.dl.ac.uk
Tue Jul 5 10:48:47 EST 1994
In article <CsH3DG.76H at usenet.ucs.indiana.edu> jgraham at bronze.ucs.indiana.edu (the End) writes:
>Has anyone used P1vir to transduce E. coli to recA- using a marker other than
>tn10(tetR) ? My host strain is already tetR and I have been trying to move
>a tn9-200 linked recA marker from DB1319 (recA938::Tn9-200). So far no
Many years ago, I did this using a double mutant, recA56 (null) and some
linked marker (nal-resistant? - I forget). Transduce with selection for
the linked marker, then screen a dozen or so transductants for eg UV
>Does anyone see any inherent problems in this strategy (eg. transducing a
>tn9 linked mutation) ?
If Tn9 is at all likely to jump after/during transduction, the answer to
your question is yes. You will need to screen your transductant - you
should anyway, of course :-) - and you may get multiple copies of Tn9
in your new strain.
Robin Walters. Robert Hill Institute, Sheffield UK.
A fact is an opinion that everyone agrees with.
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