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DD-RT-PCR Eluting Bands From Gels

John Fagan jfagan at miu.edu
Tue Jul 5 19:34:12 EST 1994

Dear DD-RT-PCR Aficionados,
We are not having great luck eluting DD products from dried gels.  What
are other!s experience with this?  If you are successful with this, what
are the specifics of your procedure?  Is it significantly better to work
with undried gels?  If so, have you shifted to labeling with 32P, or is
35S still adequate?
	John Fagan

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