In article <kirk.cunningham-050794150513 at 124.52.med.umich.edu>,
kirk.cunningham at um.cc.umich.edu (Kirk Cunningham) wrote:
> Are there any commercially available vectors for making HA-tagged proteins?
> Seems like all the references we can find have used PCR-based methods with
> the tag built into the primers.
The best way *is* with the tag built into the primers. Any pre-built vector
will require you to adapt your coding region into the tag, so you might as
well do it all by PCR.
BTW, there was a story going around that the HA antibody was erratically
unavailable. It certainly will bu$t your budget if you ever need a mg or so
of antibody. You might consider other tags for which the cell lines are
freely available (i.e. the myc tag, which is available from ATCC).
Dennis J. Templeton
CWRU School of Medicine