PCR product purifn. for sequencing

Shiao Y. Wang sywang at whale.st.usm.edu
Tue Jul 5 16:54:25 EST 1994


Roger Wiegand (rcwieg at ccmail.monsanto.com) wrote:

: I use a biotinolated primer and clean up on streptavidin magnetic beads.
: Works consistently, is fast, and can be scaled to fairly high throughput.

Who do you purchase the streptavidin magnetic beads from? Dynal worked
well for me, have you found an alternative source that is less costly?

Please let me know how Sequenase works out for you. I've been doing cycle
sequencing, but is thinking about Sequenase also. Thanks for your attention.

Shiao Wang
University of Southern Mississippi




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