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sub-unit separation

Knut Eichler eichler at insa.insa-lyon.fr
Wed Jul 6 13:59:13 EST 1994

Dear Networld,

We have a problem with protein purification. After several purification 
steps (DEAE, Gelfiltration, TSK-Phenyl) we have obtained two different 
subunits of an enzyme in the same fraction/peak. We are interested in any 
suggestions to separate them mildly, so that they keep their activities.
Thank you very much for any help!
Knut Eichler
Laboratoire de Genetique Moleculaire des Microorganismes (LGMM)
INSA de Lyon
20, avenue Albert Einstein
eichler at insa.insa-lyon.fr
Tel +33 72 43 87 06
Fax +33 72 43 87 14
live fast, work hard, die young (JJ, or so)

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