sub-unit separation

Knut Eichler eichler at insa.insa-lyon.fr
Wed Jul 6 13:59:13 EST 1994


Dear Networld,

We have a problem with protein purification. After several purification 
steps (DEAE, Gelfiltration, TSK-Phenyl) we have obtained two different 
subunits of an enzyme in the same fraction/peak. We are interested in any 
suggestions to separate them mildly, so that they keep their activities.
Thank you very much for any help!
Knut Eichler
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Laboratoire de Genetique Moleculaire des Microorganismes (LGMM)
INSA de Lyon
20, avenue Albert Einstein
F-69621 VILLEURBANNE CEDEX
France
eichler at insa.insa-lyon.fr
Tel +33 72 43 87 06
Fax +33 72 43 87 14
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live fast, work hard, die young (JJ, or so)
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