Ligase Chain reaction for RNA?
odonnell at sasa.gov.uk
Thu Jul 7 00:29:27 EST 1994
Alex Chang <lpss at unixg.ubc.ca> wrote :
>I have two spliced RNA fragments from a precursor hepatitis delta RNA,
>I'd like to know whether there is a base or two got deleted during the
>process of splicing.
>I would appreciate it if someone could give me some idea whether I could
>use the same strategy of LCR to detect it. I do have the DNA template
>(plasmid DNA) from which the hepatitis delta RNA transcribed from. I
>guess the critical part is, whether the heat stable ligase used in LCR
>could link two ajacent RNA fragment (which are bound to the DNA
>University of British Columbia
>lpss at unixg.ubc.ca
I suspect (though I don't know for certain) that DNA ligase will not ligate RNA.
However, why don't you make a cDNA copy of your spliced RNA and use that as your
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