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PCR product purifn. for sequencing -Rep

Shiao Y. Wang sywang at whale.st.usm.edu
Wed Jul 6 23:39:55 EST 1994


Scrimshaw, Brian J (SCRIMSHAWB%GRIEHFS at LINCOLN.CRI.NZ) wrote:

: Any help on alternative fast methods to get a band 118bp pure
: enough for manual sequencing are (still) much appreciated.

I suggest trying biotinylated primers and then purifying the PCR products
with magnetic strepavidin. Very fast.

Shiao Wang
University of Southern Mississippi




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