drm21 at mole.bio.cam.ac.uk
Thu Jul 7 07:57:40 EST 1994
I hope someone can help me...
I used to have a very useful protocol for doing minipreps in 96 well
plates. Now I can't find the paper, and I can't remember the authors names
or the journal, nor even the year! I've done a bit of literature searching
for it, but I just can't find it.
Does anyone out there know this method and have a reference? An outline of
the method as I remember it follows:
Grow up bugs in 1ml tubes on 96well format (available USA/Scientific Plastics).
Using multichannel pipette, transfer 100ul each to 100ul glycerol for stocks.
Tranfer another 100ul to another 96-well plate. Pellet cells
Prep DNA by a slightly modified alkaline lysis procedure. (This is the bit
that I need the paper for!
The DNA quality was fine for restriction analysis, but not great for sequencing.
Thanks for your help.....
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