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V517 strain - Size of plasmids?

Dr. Richard V. Goering rgoeri at BLUEJAY.CREIGHTON.EDU
Thu Jul 7 15:39:56 EST 1994



On 7 Jul 1994 jenfedaque at samba.cnb.uam.es wrote:

> Hi all,
> 
> I'm using the E.coli strain V517 as standard in plasmid preps.
> This strain harbors many plasmids, so it can be used to size any 
> plasmid in its circular form. The problem is that I don't have
> the reference for the strain, nor the molecular weights of the
> plasmids (I have some guess in MDa, but I would prefer the size in
> Kbp).
> 
> Does anyone know something about this strain (source, references,
> list and size of plasmids) ? I would be eternally grateful for
> this information ;-)
> 
> Thanx,
> 
> Sonia
> 
> Dept. Microbiology
> Fac. of Pharmacy
> Univ. of Barcelona (Spain)
>-----------------------------------------

The plasmids sizes (in kb) are:
	55.5
	 7.4
	 5.7
	 5.3
	 4.0
	 3.1
	 2.8
	 2 2

the reference is.... Plasmid (1978) 1:417-420.

Good Luck

Richard Goering
Dept. Med. Microbiology.
Creighton Univ. Sch. Med.
 
> 
> 



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