How to make adaptors

Antonio R. Franco bb1rofra at uco.es
Thu Jul 7 13:24:09 EST 1994


I ordered two complementary oligos to make an adaptor.
Is there any protocol to make it?

I am planning in using my PCR apparatus with the following protocol:
	-heat at 94 degrees
	-cool up to 10 degrees very slowly, with a ramp time of about 1 hour

Is this ok?



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