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Ligase Chain reaction for RNA?

Michael van Waes bi__mvw at SELWAY.UMT.EDU
Thu Jul 7 11:46:22 EST 1994

>Alex Chang <lpss at unixg.ubc.ca> wrote :
>>I have two spliced RNA fragments from a precursor hepatitis delta RNA, 
>>I'd like to know whether there is a base or two got deleted during the 
>>process of splicing. 

Kevin O'Donnell wrote:

>I suspect (though I don't know for certain) that DNA ligase will not ligate RNA. 
>However, why don't you make a cDNA copy of your spliced RNA and use that as your 
>LCR template? 
>Kevin O'Donnell
>Scottish Agricultural Science Agency     "We must combine pessimism 
>East Craigs                               of the intellect with
>Edinburgh           Tel. 031 244 8924     optimism of the will."
>EH12 8NJ            Fax  031 244 8940
>Scotland                                  Antonio Gramsci           
>odonnell at jura.sasa.gov.uk
I don't know about the thermostable ligase, but other DNA ligases (eg T4)
do ligate RNA; see Moore and Sharp in Science May 15, 1992.

Cheers    MvW

	   Michael van Waes	        |
	   Div. of Biol. Sci.	        |  "I never worry about the future, 
	   Univ. of Montana	        |       ... it comes soon enough. "
	   Phone: 406-243-5733  	| 
	   bi__mvw at selway.umt.edu	|            	Albert Einstein.

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