We are trying LDH assays from Promega as an alternative to the
chromium release cytotoxicity assay. However, we have background
problems with the LDH assay. We are using J774 macrophages
stimulated with LPS. At >1 ng/ml LPS, we get high LDH readings
on the J774 cells alone.
Does anyone know of any other cytotoxicity asays (preferably
non-radioactive) that we could use? Or why we have these
background problems? (We don't have such high background
when we use bone marrow macrophages.) Any help would be
greatly appreciated.
