On Thu, 7 Jul 1994 13:03:27 GMT,
BRIAN PETER MERRY writes:
>We need some help. We're running 1% and 2% gels in our lab using Lambda hin3
>as a molecular weight marker. We load 500 ng of lambda in our marker lane and
>we don't get resolution of the 500 bp band when prestaining the gel with etbr.
>Do we need more DNA in the marker lane? More etbr (we now use 2ul/50ml of
>gel, this used to be enough). Any comments/suggestions will be appreciated.
If my memory serves me correctly, the 564 base fragment and the 23130 base
fragment contain the 2 cos sites/sticky ends of the phage DNA and anneal
together. Heat marker to 65C for 5 mins and cool rapidly on ice before
loading to dissassociate the 2 fragments.
David A. Johnston
Dept of Zoology, The Natural History Museum, Cromwell Road,
South Kensington, London SW7 5DB. England
(tel 071 9389297, fax 071 9388754, email daj at nhm.ac.uk)