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How to recover small fragment from agaro

石塚俊治 HGF02633 at NIFTYSERVE.OR.JP
Thu Jul 7 09:17:35 EST 1994


Dear Netters
I separated the 100 bp, 50 bp, and 30 bp DNA fragments with NuSieveGTG
low melting point agarose gel. I want to subclone the 100 bp 
fragment. I tried to purify the 100 bp fragment. However, the recovery 
is very poor (less than 30 %). 
After electrophoresis, I excised the gel band of 100 bp DNA,  melt the
gel at 65 C,  did phenol extraction, and precipitated the DNA with 
ethanol (in 0.1 M NaCl). Does anybody know how to recover small DNA 
from agarose gel efficiently. Please teach me.

Sincerely yours,

================================================================
Dr.Toshiharu Ishizuka
Department of Biochemistry, Chiba University, School of Medicine
1-8-1, Inohana, Chuo-ku, Chiba, 260 JAPAN
FAX +81-43-226-2041
E-mail
QFH03407 at niftyserve.or.jp
tishizuk at twics.com
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