missing 500 bp band w/ lambda hind3

BRIAN PETER MERRY 002846m at axe.acadiau.ca
Thu Jul 7 08:03:27 EST 1994

We need some help.  We're running 1% and 2% gels in our lab using Lambda hin3 
as a molecular weight marker.  We load 500 ng of lambda in our marker lane and 
we don't get resolution of the 500 bp band when prestaining the gel with etbr.
Do we need more DNA in the marker lane?  More etbr (we now use 2ul/50ml of 
gel, this used to be enough).  Any comments/suggestions will be appreciated.

Brian Merry

More information about the Methods mailing list