missing 500 bp band w/ lambda hind3
BRIAN PETER MERRY
002846m at axe.acadiau.ca
Thu Jul 7 08:03:27 EST 1994
We need some help. We're running 1% and 2% gels in our lab using Lambda hin3
as a molecular weight marker. We load 500 ng of lambda in our marker lane and
we don't get resolution of the 500 bp band when prestaining the gel with etbr.
Do we need more DNA in the marker lane? More etbr (we now use 2ul/50ml of
gel, this used to be enough). Any comments/suggestions will be appreciated.
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