in vitro transcription

Mark Pavlick mpavlick at popserver.nih.gov
Fri Jul 8 11:01:44 EST 1994


						I want to make an RNA copy of a DNA control fragment for use as an
internal standard in competitive PCR. The fragment contains the T7
polymerase promoter. Unfortunately no-one in my immediate environment has
any experience with this technique. I would appreciate any advice regarding
problems and pitfalls. E-mail: mpavlick at pop.nih.gov



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