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P33 vs S35 for PCR sequencing

orchid phud pc-clegg at smaug.ucr.edu
Fri Jul 8 15:01:06 EST 1994

We have switched to 33P for cycle sequencing in our MJ Research PTC-100.
The company informed us several months ago, that with the thin-walled reaction
tubes used in the machine there are problems with 35S.  They said that 35SdATP 
reacts during temperature cycling to form a gas that can leak through the walls
of the tubes and make... well, a mess.  Yes, its more expensive but I'd rather not
be breathing in my work! Hope this 2 cents helps...

David Jarrell

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