RNase-free - HELP

Bohdana Badzio bbadzio at gpu.srv.ualberta.ca
Sun Jul 10 19:06:40 EST 1994


 Hello!
 What is the most reliable - and the least detrimental - anti-RNase
treatment of 
microtome or cryostate blades to be used in in situ hybridization?
How "bad" a blade's baking (eight hours, 200 c) is for its sharpness or its 
quality in general? Is incubation with hydrogen peroxide (3%) or with DEPC
(0.1%?) satisfactory? What are other known methods?
  A bit aside, what kind of RNase inhibitor is commonly used during processing
tissue sections for ISH; is it used during hybridization step, too?

  Thanks a lot for reading this and sharing your experience.

  Bohdana Badzio
  Department of Biological Sciences, University of Alberta
  bbadzio at gpu.srv.ualberta.ca
 
Bohdana Badzio,
Department of Zoology, University of Alberta
wgallin at gpu.srv.ualberta.ca



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