phenol substitute for DNA/RNA?

Winoto lab lab_winoto at maillink.berkeley.edu.
Sun Jul 10 17:19:25 EST 1994


In article <CsoL5r.JFB at crash.cts.com>
rcdana at crash.cts.com (Richard Dana) writes:

> Substitute for phenol to do DNA and RNA extractions:
> 
>         Is there a good substitute?
> 
>         The widespread use of this reagent should be stopped if someone
>         can find a safer replacement substance. 


Richard,

There is a good reagent on the market that substitutes for
phenol/chloroform on a small scale (mini-preps, restriction digests
etc) called Strataclean resin.  Check it out in your nearest Stratagene
catalog.  By the way, it does not work for RNA manipulations, only DNA.

Paul W. Diaz
lab_winoto at maillink.berkeley.edu



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