(COPY) boil page gel in TCA?

sagara IAMWCNG at MVS.OAC.UCLA.EDU
Mon Jul 11 00:01:58 EST 1994


This is a copy that has been filed with protein analysis bionet.


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Date:    Sun, 10 Jul 94 21:46 PDT
To:      proteins at net.bio.net
From:    sagara                               <IAMWCNG>
Subject: boil page gel in TCA?

Dear netters: Does anyone know why one should boil the SDS gel
in TCA (say 5% TCA) to detect the kinasing of the substrate on
the SDS gel? I came across this method recently in a paper. I
would appreciate any info on this matter either e-mailed
directly to me or to the above net. By the way the procedure
uses gamma-labelled 32-P-ATP and Histone. Of course, the
purified protein that is suspected of kinase activity is also
there in the reaction mix. Also i have concerns about the
viability of SDS gel in TCA under such harsh conditions. Please
help me. Thanks for your contribution.




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