Who can recommend me a reliable method for preparative scale
purification of a large plasmid ?
I am working with a 160 KB plasmid from a Gram + organism.
We have tried a variety of methods with very disappointing results.
All are labor intensive and give very poor yields
(5 ug / lt culture approx.), or no plasmid at all.
Does anybody have tried something like large scale plasmid
purification using pulsed field electrophoresis?
Thanks for your help.
(menendez at sun2.ruf.uni-freiburg.de)