Reamplification mtDNA, should I?

Mike Morris mike at divsun.unige.ch
Tue Jul 12 01:46:43 EST 1994


  If I were you, I would do an initial 30 cycles with primers 
further out (say 180-200 bp, and then a further 30 cycles nested 
with the primers you have used - this should give you plenty of
DNA, without serious contaminations.
     Mike
*******************************************************************
Michael Morris PhD            
Division of Medical Genetics       tel (Switzerland) (22) 702.56.94
CMU, University of Geneva          fax (Switzerland) (22) 702.57.06
Geneva, Switzerland                email mike at medsun.unige.ch                  




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