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Reamplification mtDNA, should I?

Mike Morris mike at divsun.unige.ch
Tue Jul 12 01:46:43 EST 1994

  If I were you, I would do an initial 30 cycles with primers 
further out (say 180-200 bp, and then a further 30 cycles nested 
with the primers you have used - this should give you plenty of
DNA, without serious contaminations.
Michael Morris PhD            
Division of Medical Genetics       tel (Switzerland) (22) 702.56.94
CMU, University of Geneva          fax (Switzerland) (22) 702.57.06
Geneva, Switzerland                email mike at medsun.unige.ch                  

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