Reamplification mtDNA, should I?

[Mike Morris]

  If I were you, I would do an initial 30 cycles with primers 
further out (say 180-200 bp, and then a further 30 cycles nested 
with the primers you have used - this should give you plenty of
DNA, without serious contaminations.
     Mike
*******************************************************************
Michael Morris PhD            
Division of Medical Genetics       tel (Switzerland) (22) 702.56.94
CMU, University of Geneva          fax (Switzerland) (22) 702.57.06
Geneva, Switzerland                email mike at medsun.unige.ch