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differential precipitation of PCR

Jim Owens jow at helix.nih.gov
Mon Jul 11 16:57:24 EST 1994


In article <2vrtgo$edn at strauss.udel.edu> John H McDonald,
mcdonald at strauss.udel.edu writes:
>This procedure removes unincorporated primers and nucleotides well enough
>that the DNA can be used for sequencing.  We have used it on PCR products
>that are 300 bp and larger; I don't know how much smaller you can go
>before the DNA doesn't precipitate.

I have been able to precipitate PCR products down to about 110 bp.  That
is the shortest fragment I have amplified.

Good luck,

Jim Owens



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